Analytical Lab

Features

In JNM-ECA Series (Delta V4.3) - 400 MHz- FT-NMR instrument liquid-state NMR as well as solid-state NMR measurement becomes possible. High-Resolution solid-state NMR measurement by techniques such as cross-polarization-magic angle spinning (CP/MAS) method becomes possible when you add optional attachments, such as a probe for the solid-state measurement, to the standard composition of JNM-ECA/ECX series. Multi-nuclear observation (31P-15N) measurement and measurement under variable temperature (VT) are also possible in this system.

Performance

Following operations can be carried out with this instrument:

• • 1D (1H and 13C) measurement

  • 2D (1H and 13C) measurement using COSY and DEPT

  • HMQC and HMBC observation for heteronuclear correlation spectras

  • 1D and 2D NMR measurement are performed to obtain measurement, data processing and plotting

  • Determining molecular structure and conformation of a sample and examine interactions between molecules can be achieving by 1D and 2D NMR (interpretation will be done by users only).

Applications

Determining molecular structure and conformation of a sample and examining interactions between molecules can be achieved by 1D and 2D NMR.

Sample Specifications

For liquid NMR

• The concentration required is the following

  • For 1H NMR: 5-7 mg sample  in 600 µl to 1 ml

  • For 13C NMR: 15-30 mg sample in 600 µl to 1 ml

• Sample should be completely dissolved in deuterated solvent.

For solid NMR,

• 200 - 500 mg samples are required

• The sample should be in fine powder form or in small pieces

• Sample must be non-corrosive in nature

Testing Parameters

The sample is subjected to a magnetic field of 400 MHz. The run time is dependent on the nature of the compound to be tested.

Points to be noted

• Sample must be non-toxic

• Sample should be of sufficient amount to get good results

• Bring relevant reference papers before running a sample for solid NMR

Location: SMITA Analytical Lab, TX-110 A, Dept. of Textile Technology, IIT Delhi