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NMR

Nuclear Magnetic Resonance Spectrometer


400 MHz FT-NMR, JEOL (Singapore)
Features

In JNM-ECA Series (Delta V4.3) - 400 MHz- FT-NMR instrument liquid-state NMR as well as solid-state NMR measurement becomes possible. High-Resolution solid-state NMR measurement by techniques such as cross-polarization-magic angle spinning (CP/MAS) method becomes possible when you add optional attachments, such as a probe for the solid-state measurement, to the standard composition of JNM-ECA/ECX series. Multi-nuclear observation (31P-15N) measurement and measurement under variable temperature (VT) are also possible in this system.


Performance
Following operations can be carried out with this instrument:
  • 1D (1H and 13C) measurement
  • 2D (1H and 13C) measurement using COSY and DEPT
  • HMQC and HMBC observation for heteronuclear correlation spectras
  • 1D and 2D NMR measurement are performed to obtain measurement, data processing and plotting
  • Determining molecular structure and conformation of a sample and examine interactions between molecules can be achieving by 1D and 2D NMR (interpretation will be done by users only).
Applications
Determining molecular structure and conformation of a sample and examining interactions between molecules can be achieved by 1D and 2D NMR.
Sample Specifications
For liquid NMR
  • The concentration required is the following
    • For 1H NMR: 5-7 mg sample  in 600 µl to 1 ml
    • For 13C NMR: 15-30 mg sample in 600 µl to 1 ml
  • Sample should be completely dissolved in deuterated solvent.
For solid NMR,
  • 200 - 500 mg samples are required
  • The sample should be in fine powder form or in small pieces
  • Sample must be non-corrosive in nature
Testing Parameters
The sample is subjected to a magnetic field of 400 MHz. The run time is dependent on the nature of the compound to be tested.

Points to be noted
  • Sample must be non-toxic
  • Sample should be of sufficient amount to get good results
  • Bring relevant reference papers before running a sample for solid NMR
Location: SMITA Analytical Lab, TX-110 A, Dept. of Textile Technology, IIT Delhi
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